Oxford Nanopore Technology (ONT) uses an array of nanopores to sequence DNA strands, enabling real-time base-calling. The base-called data is utilized in the ‘Read Until’ method, an advanced adaptive sequencing technique that permits real-time selective sequencing of DNA without the need for extensive library preparations. In adaptive sampling (AS), sequence data is generated and compared in real-time to a selected region of interest (ROI) within the genome. If a DNA strand’s sequence does not match the ROI, the voltage is reversed, ejecting the strand from the pore and making the pore available to read another strand. We investigated the fold enrichment one can achieve with AS, the impact on enrichment if different length and/or number of ROIs are used and its impact on flow cell lifespan. Results showed that AS significantly increased ROI coverage without affecting read quality or flow cell lifespan. The size and number of ROIs did not impact enrichment, and AS could sequence into unknown regions from known ones, albeit with reduced coverage. These findings highlight AS as a promising tool for targeted long-read sequencing.