Abstract:

Oxford Nanopore has developed a new generation of DNA/RNA sequencing technology. It is the only sequencing technology that offers real-time analysis (for rapid insights), in fully scalable formats from the pocket-sized MinION to the high-throughput, population-scale PromethION — scale nanopore sequencing to suit any experimental needs. Further, the technology can analyse native DNA or RNA and sequence any length of fragment to achieve short - to ultra-long read lengths.

Traditional methods are only able to sequence short lengths of DNA which must then be reassembled. It is therefore difficult to sequence repetitive regions for accurate genome assemblies without gaps, resolve large structural variations, or differentiate isoforms.

Nanopore sequencing is limited only by the length of the DNA/RNA fragment presented to the pore and can therefore span entire repetitive regions, resolve structural variants, and differentiate between different isoforms. The ability to sequence native DNA and RNA without the requirement for amplification, eliminates PCR bias and allows for the identification of base modifications, such as methylation, alongside nucleotide sequence.